Hansel’s Stain
Method used referenced from Sheldon, Lovell and Mathews’ “A Manual of Clinical Allergy” (published by W B Saunders Company, 1967).

Stains should be kept in amber dropper bottles and freshly made up every 2 months.

Staining Methods

1. Flood an air dried slide with eosin stain and allow to stand for 1 minute.
2. Add an equal volume distilled water for 1 minute.
3. Drain and flood slide with distilled water until all the stain is removed.
4. Flood slide with 95% ethanol and then drain.
5. Stain immediately with methylene blue for 1 minute.
6. Add an equal volume distilled water until all the stain is removed.
7. Flood slide with distilled water until all the stain is removed.
8. Flood slide with 95% ethanol and air dry.
9. Examine under oil immersion.

Technique

1. Small child to sit on mother’s lap.
2. Explain the procedure to the mother and child.
3. Using a clean, dry glass rod gently remove mucus from inner surface of nostril.
4. Spread evenly over surface of clean glass slide.
5. Label and date slide.

NOTE:
An alternative way of collecting a sample would be for the patient to blow their nose on wax paper.

Nasal Eosinophils Interpretation

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